Lab Technician, Quality Control, Alachua, FL

    • Job Tracking ID: 85430-275054
    • Job Location: Alachua, FL
    • Job Level: Any
    • Level of Education: Any
    • Job Type: Full-Time/Regular
    • Date Updated: 10/08/2018
    • Years of Experience: Not Applicable
    • Starting Date: ASAP
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Job Description

Position Description/Summary:

Brammer Bio, LLC is a dynamic gene and cell therapy contract development and manufacturing organization that is looking for leaders to bring the highest quality processes, products and testing to our clients. Our staff members; are committed to making a difference in our organization, for our client partners and the patients we serve. We seek individuals with proven competencies and strong character to help lead our organization now and into the future.

The Laboratory Technician will work in the QC cGMP compliant Laboratories to develop, qualify, and conduct cell-based, nucleic acid-based, protein-based, and chemistry-based assays that support quality control of biopharmaceutical products being developed and manufactured at Brammer Bio under contract. Duties include performing routine assays and testing including but not limited to PCR, quantitative PCR, agarose gel electrophoresis, SDS-PAGE, immuno blotting, ELISAs, immunocytochemistry, and infectivity and reporter gene based cell assays using insect or mammalian cell lines. The Laboratory Technician will also troubleshoot and optimize assays under the supervision of the GMP QC Manager/ Research Coordinator. Functions also involve maintenance of cell lines used in the QC laboratories, maintenance (routine and preventive operation) of equipment, and preparation and purification of commonly used biological reagent and chemical supplies. The Laboratory Technician will be expected to technically interpret and review data produced from the assays they run.

Essential Responsibilities:

Qualify and evaluate the results of qPCR assays, infectivity assays including TCID50, and other assays related to the safety and release testing of intermediate and final biological products (toxicological and clinical materials), and their stability, manufactured by Brammer Bio. These assays must be performed according to written procedures and in compliance with cGMP regulations. 35%

The Laboratory Technician will qualify and perform and evaluate the results of qPCR assays, gel electrophoresis assays, Western blot assays, quantitative protein assays (i.e., Bradford, Lowry, BCA, etc.), ELISAs, spectrophotometric assays, cell viability assays, infectivity assays, and other assays related to the safety and release testing of process development intermediate and final biological products (toxicological and clinical materials), and their stability, manufactured by Brammer Bio in the Process Development labs. 25%

Manage reagents and material supply chain, maintain equipment within the QC testing lab. Maintain sample inventory system including daily database entries. 10%

Perform cleaning, calibration, preventive maintenance of equipment at specified intervals. 10%

Maintain complete, contemporaneous and accurate records of all work performed. Maintain equipment records as required by written procedure. 10%

Maintain appropriate in-house assay standards including inventory and database of each standard’s performance in each assay. 5%.

Other duties as assigned by supervisor. 5%

Physical Demands:

While performing the duties of this job, the employee is frequently required to stand; repetitive use of fingers/hand to pipet.

Specific vision abilities required by this job include close vision, and ability to adjust focus.

This position requires use of face mask, and eye protection, and other PPE.

Benefits:

Brammer Bio offers an attractive benefit package to all full time employees which includes; medical, dental, and vision coverage, company paid Disability and Life Insurance benefits, and a 401(k) Plan with a generous company match. All full time employees are also provided with paid time off, paid company holidays, share appreciation rights, and an incentive bonus plan.

Brammer Bio is an Equal Opportunity Employer and participates in eVerify.

Experience and Skills

Required:

A high school diploma and a minimum of two years of work experience. Appropriate vocational/technical training may substitute at an equivalent rate for required experience or exempt by Manager approval.

Preferred:

Bachelor’s Degree in appropriate area of specialization.

Laboratory work experience in the fields of biotechnology, immunology, molecular biology, cell biology, or virology are preferred for this position.

Hands on experience with assay development and assay qualification in a cGMP compliant Quality Control laboratory for the performance of a variety of biological assays, including cell-based (viability, tissue culture, infectious titer), nucleic acid-based (restriction digests/gel electrophoresis, PCR, qPCR, DNA sequence analysis, spectrophotometric assays), protein-based (BCA, SDS-PAGE, Western, ELISA, spectrophotometric assays), and chemistry-based assays (pH, Osmolality, etc); including technical understanding of assay principles are highly desired for this position.